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A total of 27 students at the University of Granada were recruited by standard procedures in May 2008.
All zebrafish work was conducted in accordance with relevant European Union regulations for animal experimentation (Umeå regionala djurförsöksetiska nämnd Dnr A13 15).Maintenance and breeding of zebrafish: Zebrafish were maintained by standard procedures in the Umeå University Zebrafish Facility.
Antibodies were purified and conjugated by standard procedures in the Weissman laboratory, unless purchased commercially as stated above.
Sample hematopathology slides with known diseases were identified from the teaching collection of the Pathology Department at UC Davis Medical Center, and were prepared by standard procedures in the hematopathology laboratory at the University of California, Davis.
Fungal culture and identification were performed by standard procedures in a clinical microbiology laboratory.
This was then used to generate monoclonal antibodies by standard procedures in our monoclonal antibody service.
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For immunohistochemical staining, tumor tissue sections (5 mm) were prepared and deparaffinized in xylene, hydrated by standard procedures described in our previous study.
Signals from the electrode wires were amplified and filtered by standard procedures described in the previous studies.
HI assays were performed by standard procedures [20] in round-bottom polystyrene microtitre plates (Nunc, Denmark) using 1% chicken red blood cells.
Liver samples were fixed with neutral-buffered formalin, processed by standard procedures, embedded in paraffin, sectioned, and stained with hematoxylin and eosin for light microscopy examination.
Synthesis of cDNA was performed with 2 μg of total RNA using the SuperScript II polymerase and oligo (dT 12 -18 primers by standard procedures (Invitrogen) in a final volume of 20 μl.
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