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Parasites were processed by standard procedures for immunofluorescence assays.
However, when these embryos were processed by standard procedures for WISH, the eGFP antisense probe labeled not only the eGFP-expressing cells but also the RFP-positive cells (Figure 2A D).
Post fixation was performed in 2% osmium tetroxide for 2 h followed by standard procedures for dehydration.
Equal amounts of protein from the two different cell populations are combined, followed by standard procedures for sample preparation for MS analysis.
These samples had been tested previously by standard procedures for the diagnosis of neuroblastoma using fluorescence in-situ hybridisation (FISH) [ 10].
Plasmid pEGFP-IRES2-Vim that encode EGFP and human vimentin divided by internal ribosome entry site (IRES) was constructed using vector pEGFP-IRES2 (Clontech, USA) by standard procedures and used for restoration of VIF in MFT-16 cells where indicated.
The plasmids were transformed into the yeast strain Y2HGold (Clontech) by standard procedures, and we screened for positive interactions using X-α-Gal and His as markers.
Genetic analyses were performed by using standard procedures for mating, diploid selection, sporulation, and tetrad dissection.
These multiple imputed datasets are analyzed by using standard procedures for complete data.
Cloacal swab samples were collected from both dead and apparently healthy chickens on farms and tested by using standard procedures for virus isolation (3 ).
Although our conclusions are strengthened by the standard procedures for retrieval, assessment of relevance, and statistical processing in this systematic review and meta-analysis, a number of potential limitations should be taken into account.
