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All primers were gel purified using 20% denaturing polyacrylamide gel electrophoresis and radiolabeled by standard procedures at the 5′-end using [γ-P]ATP and T4 polynucleotide kinase.
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The 18650-type batteries produced by standard procedure at LG Chem.
DNA was isolated by standard procedure at the WTCRF Genetics Core, Western General Hospital, Edinburgh from 1,071 individuals.
Sterility, absence of endotoxins, pH, osmolality and residual solvents were determined by standard procedures routinely performed at the PET Centre of the Vienna General Hospital/Medical University of Vienna and follow the respective monograph in the European Pharmacopoeia [25].
Serum samples were separated by standard procedures and stored at −20 °C for subsequent analysis.
This strain was transformed by standard procedures and cultured at 37 °C in Luria-Bertani broth or agar (17) containing, where appropriate, 100 μg/mL ampicillin.
Genomic DNA was extracted from peripheral blood monocytes by standard procedures (Qiagen) and genotyped at either the Wellcome Trust Sanger Institute on the Human 610 Quad Custom Array (594 398 SNPs and 66 049 CNVs), or the SNP&SEQ Technology Platform at Uppsala University, using the Sentrix Human Custom 1.2 M array (1 115 839 SNPs and 80 128 CNVs).
Sample hematopathology slides with known diseases were identified from the teaching collection of the Pathology Department at UC Davis Medical Center, and were prepared by standard procedures in the hematopathology laboratory at the University of California, Davis.
Normalized cDNA libraries from the polyadenylated RNA fraction from nine of the mixed stage nematodes were constructed at the GSC at Washington University by standard procedures and sequenced on the Roche 454 FLX platform producing 320,000 to 540,000 reads.
Skeletal muscle mitochondria were isolated at 4 °C in Chappell Perry buffer (CP1; 100 m m KCl, 50 m m Tris, 2 m m ethylene glycol tetraacetic acid (EGTA), pH 7.1 at 25 °C) by standard procedures.
Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were measured in the Institute of Clinical and Laboratory Medicine at the University Hospital Heidelberg by standard procedures.
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