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SDS-PAGE was performed by standard procedures according to the manufacturer's instructions (Invitrogen, Karlsruhe, Germany).
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Recombinant vesicular stomatitis virus VSV -pseudotyped lentivirus VSV -pseudotypedained by standard procedure, according to Tiscornia et alentiviral
ALP staining was performed by a standard procedure according to the manufacturer's instructions (Sigma-Aldrich Chemical Company, USA).
All US teleradiology examinations have to be performed by sonographers according to a standard procedure, according to Prof. Jan Labuscagne, President of the International Society of Radiology.
Preparation of chromosomal and plasmid DNA, and transformation were carried out by standard procedures or according to supplier protocols (Qiagen, Frederick, MD, USA).
Bacterial cultures were identified morphologically and biochemically by standard laboratory procedures according to the Manual of Methods for General Bacteriology in the bacteriological laboratory of Sumy State University.
All DNA manipulations were performed according to standard methods [79] and all media were prepared by standard procedures [80].
DNA was extracted from an overnight culture by standard alkaline lysis procedures according to Sambrook [ 29] and used for sequencing initially from the 5' end using a SP6 primer and then from the 3' end with a T7 primer in cases where an SSR was not detected at the 5'end.
Virus isolation and detection by immunoperoxidase staining was performed on serum samples and/or organs of all pigs in this study by standard procedure of the institute according to Uttenthal et al. [ 20].
Sequences were confirmed by standard procedures.
Plasmids were constructed by standard procedures [37].
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