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After CT scan, we performed lavages of the ETT inner surface injecting 5 ml of sterile saline solution, and the collected fluids were cultured by standard microbiological tests.
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We compared the results obtained using a novel, culture-independent polymerase chain reaction/electrospray ionization-mass spectrometry technology with those obtained by standard microbiological testing and evaluated the potential clinical implications of this technique.
The isolated organisms were identified using standard microbiological tests.
307 bacterial isolates were classified based on morphology, Gram-staining and standard microbiological tests.
All strains were characterized using a phase-contrast microscope and standard microbiological tests (Gram staining, Oxidase test).
Part of the CSF sample from all animals was used for confirmation of the presence of microorganisms using standard microbiological tests (10 15 µl CSF).
The species identification of the isolates was based on standard microbiological tests: colony morphology, acid-fast staining, and biochemical tests [ 6].
Stool samples were pre-screened using standard microbiological tests to rule out bacteria and parasites and stored at −20 C Enzyme immunoassay (EIA) were performed on stool specimens.
Hokkaido regional accreditation requires natural cheese to be negative for coliform and L. monocytogenes in 25 g by the standard microbiological test (http://www.pref.hokkaido.lg.jp/ns/shs/06/ninshou/cheese-kijyun.pdf).
Registration of the origins of isolate cultures as well as standard microbiological testing, including oxacillin susceptibility testing (by CLSI) was performed in Cuba.
PCR results were concordant with standard microbiological testing.
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