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Genomic DNA was extracted from peripheral blood by standard methods or saliva samples using Oragene collection kits according to manufacturer's instructions.
No putative self-replicating plasmid could be isolated by standard methods or by following a procedure for the isolation of total genomic DNA.
Organs were fixed with 10% formalin and embedded in paraffin by standard methods or were embedded without fixation in Optimal Cutting Temperature (OCT) embedding compound for frozen sections.
Other studies have investigated the association of hepatic fibrosis and clinical course after portoenterostomy, with poor outcome reported for children with advanced fibrosis, either quantified by standard methods or aided by computerized morphometry [ 18- 21].
Phalloidin-stained fibers were visualized by confocal fluorescence microscope equipped with narrow band filters, by standard methods or by the Zeiss Axio Observer Z.1 microscope platform in conjunction with Zen 2011 Blue imaging software (Carl Zeiss)(insets in Figure 5A).
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Different custom biobricks luxABCDE operon, gfp, gusA, lacZ were when cloned into pBAV1K-T5 vector by standard methods [13] or overlap extension PCR cloning [22].
Total genomic DNA was extracted by standard methods from peripheral blood lymphocytes or muscle biopsies.
Donor cells were isolated by standard methods mainly using collagenase II or trypsin/EDTA [ 41].
The results are compared with those available in literature or calculated by standard methods using binary interaction parameters.
All isolates were identified by standard methods and confirmed by using Vitek or API products (bioMerieux Vitek, Inc., Hazelwood, MO).
DNAs were extracted by standard methods from pellets of cell lines, or from frozen primary tumours.
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by microscopic methods or
by direct methods or
by immunohistological methods or
by other methods or
by different methods or
by numerical methods or
by gravimetric methods or
by serological methods or
by invasive methods or
by radiometric methods or
by seismic methods or
by phenotypic methods or
by quantitative methods or
by immunologic methods or
by immunochemical methods or
by standard formulations or
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