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Immunohistochemistry was carried out by standard methods on a DAKO Autostainer (Dako, Ely, UK), using its proprietary kit, the DAKO Envision FLEX/HRP Detection System.

The X-ray diffraction analysis (XRD) of the samples was realized by standard methods on the X-ray diffractometer "Stoe" at 45 kV and 33 mA (CuKα irradiation) [4, 5].

Western blots were carried out by standard methods on proteins transferred to PVDF using TransFi (Invitrogen).

C. elegans were grown by standard methods on nematode growth media (NGM) at 20°C with E. coli OP50 as a food source [6].

All biochemical parameters were determined by standard methods on a Roche-Modular Autoanalyzer (Milan, Italy).

Caenorhabditis elegans, N2 Bristol wildtype strain, was cultured by standard methods on Luria Bertani agar plates at 20°C [ 44].

Gross energy and proximate composition of Chaetomorpha sp. cultured at SCORL were analyzed by standard methods based on AOAC (2012) by Central Laboratory (Thailand) Co. Ltd., Bangkok, Thailand.

The dissected bands were purified by standard methods and sequenced on a 3500 genetic analyzer which is mentioned previously.

Constructs were transformed into competent E. coli DH5α by standard methods and plated on LB plates with ampicillin (50 μg ml−1).

C. elegans was cultured by standard methods (Stiernagle, 2006) on the surface of NGM 1.5% agar Petri dishes of 5.5 cm diameter.

Synchronized populations of wild-type and mutant nematodes were prepared by standard methods and cultivated at 20°C on NGM agar containing E. Coli (OP50) [32].