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In the current paper, we describe the production of human recombinant PPT1 (the defective enzyme in INCL) by standard methods in Chinese Hamster Ovary (CHO) cells.
Therefore, once the number of neighbors per data point K is chosen, the optimal weights (W_{ij}) and coordinates Y i are computed by standard methods in linear algebra.
Viral proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred to a Hybond-P membrane (Amersham-Pharmacia) by standard methods, in at least two independent experiments.
The culture strains were grown in 10 cm Petri dishes and cultured by standard methods in an atmosphere of 93% N2, 4% CO2, 3% O2 at 37°C as described previously [20].
Both viruses were propagated and titrated by standard methods in embryonated chickens eggs (ECE) [ 14].
All other laboratory tests were done by standard methods in each clinic.
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We developed cells on nitrocellulose filters (5 × 10 cells per 5 cm filter) saturated in PDF buffer by standard methods, detailed in [ 18].
DNA was extracted from peripheral blood lymphocytes by standard methods, resuspended in Tris-EDTA buffer (10 mM Tris, 1 mM EDTA), and frozen until use.
Synthesis of gold nanoshells was conducted by standard methods described in detail elsewhere.
pJFRC51 was generated by standard methods described in Pfeiffer et al. (2010).
Tests for common phytochemicals were carried out by standard methods described in Practical Pharmacognosy by Kokate and Khandelwal [ 6, 7].
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