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Core OS was isolated by standard methods from Westphal purified LOS.
This approach is compared with the numerical solution by standard methods from the literature and by well-established numerical solvers for ODEs.
N-terminally FLAG-tagged Ago2 in pcDNA 3.1 was derived by standard methods from a cDNA clone (ATCC; RefSeq NM_012154).
A LCL, which was established by standard methods from a Japanese female subject, was maintained and cultured as described previously [34].
Total genomic DNA was extracted by standard methods from peripheral blood lymphocytes or muscle biopsies.
Total genomic DNA was extracted by standard methods from peripheral blood lymphocytes.
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To examine average clone lengths, we isolated plasmid DNA by standard methods for ~50 randomly selected clones from each pDONR222 library.
Lipid profiles were determined by standard methods (kits from Roche Diagnostics; Denka Seiken, Japan; and Asahi Kasei, Japan).
Wright's staining was by standard methods, using stain from Harleco (#740, purchased from VWR, West Chester, PA, USA).
Microbial genomic DNA was isolated by standard methods using a kit from Zymo Research (Irvine, CA, USA) as per the manufacturer's instructions.
where for the coefficients may be determined by standard methods through the moments resulting from (2.5).
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