Sentence examples for by standard methodologies using from inspiring English sources

Immunoblotting was by standard methodologies, using whole-cell, SDS lysates, 3-8% Tris-acetate gels and blotting overnight at low voltage.

The methods derived from standard methodologies used by other investigators [ Crawley, 2007].

SSADM is the standard methodology used by the UK government for the development of their information systems.

FPLC is a standard methodology used to separate lipoproteins by size [ 15].

In situ hybridization by standard methodology was used to identify which gene(s) was being mis-expressed from an EY element when the genome annotation was ambiguous.

Serological responses to CS repeats (anti R32LR) were assessed by standard ELISA methodology using a plate adsorbed R32LR antigen with a standard reference antibody as a control with concentrations reported as EU/mL.

COS-7 cells were electroporated by standard methodologies.

including subtyping, grading and staging by standard methodologies [4], [21].

After cDNA synthesis by standard methodology, quantitative PCR was performed using the ABI Prism 7900 sequence detector system (Applied Biosystems), whereby 50 ng of cDNA was mixed with Taqman PCR mastermix (Applied Biosystems), QuantiTect primers (Qiagen, Crawley, UK), and SYBR Green I (Invitrogen, Paisley, UK), and the relative quantification was assessed for the KC and MCP-1 genes.

P. vivax was initially diagnosed by standard methodology and detected retrospectively by use of broad-range PCR and electrospray ionization mass spectrometry using a panel of primers designed to detect vector-borne pathogens.

SDS PAGE, blotting and immunodetection were all by standard methodology.