Sentence examples for by standard curve methods from inspiring English sources

Titer levels were derived from ODs by standard curve methods.

The caspase 3 activity in each sample solution was calculated by standard curve method.

The mRNA transcripts were quantified by standard curve method.

Absolute quantification was performed by standard curve method using PCR product-containing plasmids listed in supplementary material Table S2.

Data generated from real-time RT-PCR were analyzed by standard curve method for relative quantification of gene expression [ 60].

qPCR was carried out by standard curve method using SYBR Green with primers for GFP and RP49 as a control.

Real-time RT-PCR analysis was performed using the MyCycler (Bio-Rad, Hercules, CA, USA) with SYBR Green kits (Bio-Rad) by standard curve method as previously described.

Amplification was performed with quantitative real-time PCR, by standard curve method, with specific Taqman probes (from Applied Biosystems) for the targeted gene mouse Coq9 (Mm00804236_m1), Coq7 (Mm00501588_m1), Coq6 (Mm00553570_m1), Coq5 (Mm005018239_m1), Adck3 (mM00469737_m1) and the mouse Hprt probe as a standard loading control (Mm01545399_m1).

Relative gene expression was determined by the 2-ΔΔCT or standard curve methods in a SDS software version 2.0 (Applied Biosystems).

Quantitative real-time PCR was performed with the SyberGreen reagent (Bio-Rad) in triplicates and analyzed by the standard curve method standardized to the housekeeping gene beta actin[ 23, 24].

mRNA abundance was evaluated by the standard curve method and the value of Rev-erbα obtained was divided by the GAPDH value to obtain a normalized value.