Sentence examples for by standard cloning methods from inspiring English sources

All plasmids were assembled by standard cloning methods and confirmed by DNA sequencing.

The genes encoding the cellulases, scaffoldins, dockerin-replaced cellulases, and multifunctional cellulases were constructed and cloned by standard cloning methods [ 39].

Derivatives of pAO01 containing spt16 -G132D and spt16 -T828I/P859S alleles were generated by standard cloning methods and verified by sequencing.

The expression plasmid of EBP2 was cloned into pcDNA3.1 vector with a FLAG tag at the C-terminus and c-Myc was cloned into pcDNA3.1 vector with an HA tag at the N-terminus by standard cloning methods.

Up- and downstream flanks were fused to the floxed dominant resistance markers by standard cloning methods, and re-amplified using the forward primer of the upstream flank (HXT xuf) and the reverse primer of the downstream flank (HXT xdr).

Constructs were generated in the pcDNA3.1 vector by standard cloning methods, to express fusion proteins composed of a nuclear localization signal, yellow fluorescent protein, and 200 amino acids of the lamin B1 C-terminal region.

All vectors were constructed by following standard cloning methods and verified by restrictive digestions.

Since TALE repeats can be assembled cheaply by using standard cloning methods in the laboratory (Zhang et al., 2011; Huang et al., 2011), combined with the low cost of maintaining X. tropicalis, our findings suggest that TALENs offers a relatively cheap and efficient means of generating targeted mutations in genes in this system.

The sensitivity of ultra-deep sequencing in its current platform for detection of low-level viral variants at levels 0.1 to 1% has been confirmed by used of standard cloning methods [9], [18], [24], [25].

All the plasmids were constructed using standard cloning methods (restriction and ligation).

Plasmids were generated using standard cloning methods.