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Although untagged, ΔNArm1 128 can be distinguished from endogenous Armadillo by staining with both the "N27" (N-terminal, N27A1) and the "Arm" (central Armadillo repeats) antibodies, as the latter will identify both endogenous and overexpressed proteins (Figure 1).
Necrotic cells were identified by staining with both PI and BF dyes.
In mice, heterochromatin can be clearly visualized by staining with both DAPI and HP1 α, a heterochromatin marker [ 12, 18].
GFP-Dia-FL was expressed in stage15 embryonic tracheal cells under btl-Gal4 and localization of the endogenous protein (in the S region) as well as the expressed protein (marked with GFP), was followed by staining with both anti-Dia (red, A′ ) and anti-GFP (green, A″ ) antibodies.
Interestingly, the quantitative analysis of the distribution of AGT in cells expressing the G170R-Mi/S81L-Ma conshowed showed a significant peroxisomal localization as highlighted by staining with both the anti-AGT and the anti-FLAG antibody (Pearson coefficient of 0.55 and 0.53 for the colocalization of the peroxisomal marker with anti-AGT or anti-FLAG, respectively) (Fig. 6).
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Mature vessels were defined by staining with antibodies to both LH39 and to CD31, using double immunohistochemistry, whereas immature vessels stained only for CD31.
We measured LMP both by staining with the lysosomotropic agent Acridine Orange (AO) and by immunoblot detection of CatD in cytosolic fractions.
To determine whether loss of Arf could facilitate an earlier initiation of the hyperplastic switch, we examined the percentage of hyperproliferative islet lesions (as determined by staining with the Ki67 proliferation marker) at both 3- and 5-weeks of age in RIP-Tag2; Arf+/+ and Arf−/− mice.
Purity assessed by staining with anti-CD20 or anti-CD4 was ≥95% for both populations.
Mitochondria were visualized by staining with rhodamine 123 (Sigma).
The purity of myoblasts was confirmed by staining with anti-Pax7 antibody.
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