Sentence examples for by staining the slides from inspiring English sources

Ki67 was detected by staining the slides with Ki67 antibody (#M7240, Dako, Glostrup, Denmark) followed by exposure to Alexa Fluor 680 -tagged secondary antibody (Invitrogen Inc., Carlsbad, CA).

Late liver stage parasites expressing PfMSP-1 were counted by staining the slides with an anti-PfMSP-1 mAb and fluorescently labeled secondary antibody.

B7-H3 and p-Stat3 protein expression were detected by staining the slides with a B7-H3 AF1027dy (#and027) and p-Stat3 antibody (#9131) (both purchased from R&D, Minneapolis, MN, USA), followed by exposure to Alexa Fluor 680-tagged secondary antibodies (Invitrogen Inc., Carlsbad, CA, USA).

The optimal antibody titers were assessed by staining TMA slides and choosing the concentration with the most discriminatory power, with the intensity ranging from negative-to-strong staining among different cases on the same slide.

Visualization of the potential cortical target layers by staining consecutive brain slides with the laminar markers revealed that TCAs preferentially target the hollow of the layer IV barrels (Fig.  3 C, asterisks) and, to a lesser extent the layer V/VI border.

Staining reproducibility was verified by staining five new slides of previously stained Ki-67 cases for comparison purposes.

After air drying, heat fixation, and gram staining, the slides were examined by light microscopy.

Evaluation of apoptosis by TUNEL staining on slides was performed at the Rodent Histopathology Core Laboratory of the Dana-Farber/Harvard Cancer Center (Boston, MA, USA).

The mount was rehydrated in increasing dilutions of ethanol in distilled water (70%, 50 %, 30 %, 10 0%, 10 minutes each) and then stained by placing the slide in Carmine Alum stain over night.

Sections on glass slides (approximately 2 μm in thickness) were stained for LM by incubating the slides in 0.1% toluidine blue (Sigma-Aldrich) for 10 s on a Combimag RCT hot plate stirrer (IKA-Werke, Staufen, Germany) at 70°C.

Staining was terminated by washing the slides with running tap water for 2 minutes, and then rinsing in distilled water for a further 2 minutes.