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Proliferating cells were tested by staining of section with mouse anti-Ki-67 antibody (Neomarkers/Labvision) and angiogenesis was visualized by staining with mouse anti-CD34 antibodies (Neomarkers/Labvision, Fremont, CA, USA).
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The specificity of immunohistochemical stainings of the different anti-TRAIL-R mAbs was determined by staining of sections of formalin-fixed, paraffin-embedded pellets of CV1 cells transfected with pCDNA3.1-based expression vectors for TRAIL-R1 to TRAIL-R4 as described previously [ 22].
The clumps contained accumulations of FN and Col IV as shown by staining of the sections.
The majority of mononuclear cells were of the macrophage lineage as confirmed by staining of serial sections with CD68.
Bone destruction was assessed by staining of paw sections with hematoxylin and eosin to assess erosion scores.
This was accompanied by a diminished accumulation of starch in stamens of jai1-1 in comparison to WT stamens as visualized by staining of fresh sections.
The intratumoral blood vessels in the most intensive neovascularization areas were quantified by staining of the sections with haematoxylin and eosin followed by silver.
The usefulness of the evolved binders was verified by staining of plant sections, where they performed better than the xyloglucan-binding module from which they had been derived.
Localization was confirmed by staining of consecutive sections with α-smooth muscle actin (α-SMA) and Von Willebrand for smooth muscle and endothelial cells, respectively.
Morphological changes of the brain and eyes in fish treated with 10 ng nestin MO were further evaluated by staining of the histological sections.
Histomorphometric parameters for osteoclastogenesis were analyzed by staining of femoral bone sections with anti-TRAP Ab as mentioned above.
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