Sentence examples for by stable integration of from inspiring English sources

Prolonged gene silencing by stable integration of a siRNA expression vector is currently only possible in vitro.

Recombinant protein expression in mammalian cells is mostly achieved by stable integration of transgenes into the chromosomal DNA of established cell lines.

The W(AeR) strain was obtained by stable integration of the AeCPR under the control of the GAL10-CYC1 promoter into the W(N) genome.

These effects can be avoided by stable integration of single copies of each expression vector into established loci in the mouse genome.

Knock-down cell lines were produced by stable integration of shRNA producing plasmid vector, pLVX-shRNA2 (Clontech), using a previously characterized human Snail2 sequence (5′-GGACCACAGTGGCTCAGAA-3′) [8], also present in dog.

Interestingly, increasing the gene dosage of PTL-1 by stable integration of a PTL-1 transgene also resulted in a higher frequency of abnormal neuronal structures in young transgenic animals compared with wild-type.

This cell line was originally generated by stable integration and amplification of a human FRβ cDNA expression construct in CHO-K1 cells [ 34].

Earlier studies also demonstrated that gene silencing by stable integration can be triggered by the presence of multiple transgene copies in transgenic plants with varying degrees of target reduction (Waterhouse et al. 2001; Kerschen et al. 2004).

The WSA206 promoter functions were defined by stable integration in Arabidopsis using GUS tag.

Cells were selected for stable integration of the lentivirus by incubation with 1 μg/mL puromycin (Wako, Osaka, Japan).

This, other than the changes in the operator heptamer, led to a reduction of background activity most pronounced after stable integration of the Ptet-T2 by a retroviral vector.