Sentence examples for by stable expression of from inspiring English sources

As our comparative model, we used a pair of human colon cancer cell lines: the mutant HCT-C18(TS-) subline in which the TS protein had been rendered marginally active and the TS over-expressing subline, HCT-C18(TS+), created by stable expression of a human TS cDNA plasmid.

Sustained transcriptional modulation can be obtained by stable expression of CRISPR components, which enables directed reprogramming of cell fate.

Similarly, induction of WNT11 expression in MDA-MB-231 cells by hypoxia was reduced by stable expression of HIF-1α shRNAs (Fig. 3B; left panel).

To test whether PML RING tetramerization is also important in cellulo, we explored NBs formation and PML sumoylation by stable expression of the mutants in immortalized Pml−/− fibroblasts (Fig. 4).

A macaque CD4 + adherent cell line was generated by stable expression of the human CD4 gene in a rhesus macaque mammary tumor cell line, CMMT.

A rat oligodendroglial-derived cell line was used for a stable knock-down of eIF2B5 followed by stable expression of mutated eIF2B5 R195H) cDNA.

Chloroplast targeting of PGR7 was confirmed by transient expression of a GFP fusion protein and by stable expression and subcellular localization of an epitope-tagged version of PGR7.

The phenotypes of siRNA-treated cells were largely rescued by the stable expression of corresponding cDNAs refractory to each siRNA (Supplementary Fig. 2a f), further confirming that the above effects were not due to unexpected side effects.

By contrast, stable expression of TLR-2 or TLR-5 in HEK293 cells dramatically changes the capability of H. pylori to activate NF-κB, IL-8 and TNF-α as shown by luciferase reporter, ELISA and immunofluorescence assays.

To assure that the RNAi pathway was activated by the stable expression of shRNAs and did not interfere with HIV-1 replication leading to off-target effects, we used scrambled shRNA (shSCRAM) as control.

Targeted lines were selected by mobilizing and linearizing the original donor using hsFLP and hsI-SceI and crossing these virgin to eyFLP [40] males so that reintegration can be detected in the progeny by the stable expression of the white+ reporter.