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The initial number of colony forming units (cfu) for each experiment was determined by spreading samples from serial dilutions on KMB.
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Individual NWs for TEM observations were prepared by ultrasonicating the samples in ethanol for 5 min, followed by spreading drops from the suspension onto a holey carbon/Cu grid.
The details of the layers preparation by spreading from solution were described elsewhere [ 17].
Confirmation of presumptive E. coli O157 H7 was carried out by spread-plating 100 μL samples from each turbid tube onto tellurite-cefixime Sorbitol MacConkey agar plates (Becton Dickinson, Sparks, MD) supplemented with 200 μg mL−1 ampicillin.
Pulmonary alveolar echinococcosis is caused by hematogenous spreading from hepatic lesions [ 178].
Specifically, atypical samples spread away from major population clusters and related samples cluster toward respective subpopulations.
The three main geographic locations of sampling (Metropolitan area of São Paulo, Ribeirão Preto and São José do Rio Preto) were coded as multistate characters and the most parsimonious description of geographic spread was obtained by MCMC sampling from the plausible set of trees using the procedures outlined above.
The jaws are commonly affected sites, mostly by direct insemination or spreading from nearby tissues.
The preliminary results released by the state last week were based on soil samples from 146 additional homes spread over a two-square mile area stretching out from the plant and into Boyle Heights, Maywood, Huntington Park and East Los Angeles.
By now biopsy sampling from melanoma (which may increase the risk of spreading metastases [ 2]) is the main method for making a precise diagnosis of the malformation.
These effects are caused by the nerve fiber bundles spreading from the optic nerve [ 43].
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