Sentence examples for by spin chromatography using from inspiring English sources
Unincorporated isotope was removed by spin chromatography using a Sephadex G-25 MicroSpin™ column (Pharmacia,,,,,,, London, UK).
The 15.7 kD protein was enriched by micro-spin column chromatography using a Q HyperD® spin column (Ciphergen Biosystems, CA).
Labeled probes were purified by spin chromatography on Sephadex G-25 columns (Roche).
Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix, and the procedures were performed according to the manufacturer's instructions.
After addition of competitor metals using 100X stock solutions, the aliquots were incubated at room temperature for 15 min. The adenovirus-bound and free 99mTc were separated by gel chromatography using micro spin columns (Bio-Rad; Hercules, CA), and quantified using a NaI (Tl) γ-counter (PerkinElmer Inc.; Waltham, MA).
Nucleotides and unincorporated P were removed by gel chromatography using Micro Bio-Spin 30 columns (Bio-Rad Laboratories, HerCAles, CA, USA).
Total RNA was extracted from cells at low passage number, as described in Table 1, using TRIZOL reagent (Life Technologies) and further purified by column chromatography using a Qiagen RNeasy spin column (Qiagen).
Hybridized DNA probes were radiolabeled with γ-P-ATP in the presence of T4 polynucleotide kinase (Invitrogen Corporation) and purified by chromatography using a Sephadex G-25 spin column (Roche).
