Sentence examples for by spectrometric analysis using from inspiring English sources

Protein concentrations of enzyme extract were determined by spectrometric analysis using Coomassie Brilliant Blue G-250.

Western blot analysis was applied to two identified markers to validate the expression of intact proteins by an antibody-based approach and observed by mass spectrometric analysis using expression of digested peptides.

10 Protein extracts were analysed by acid urea-polyacrylamide gel electrophoresis (AU-PAGE) followed by mass spectrometric analysis using matrix assisted laser desorption ionisation-time of flight tandem mass spectrometry (MALDI-TOF/TOF MS).

Plasma proteins were analyzed by mass spectrometric analysis using a one-dimensional (1-D) separation approach as described below [ 12].

To further explore possible roles for Nrbp1 within mammalian cells, we performed tandem affinity purification followed by mass spectrometric analysis using a FTAP-tagged human NRBP1 cDNA expression plasmid in mouse ES cells (E14J; 129P2/Ola).

Gel slices were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK).

The eluate was monitored by absorbance at 214 nm (4), and the peak fractions were collected and subjected to mass spectrometric analysis using a Voyager-DE MALDI-TOF spectrometer (Applied Biosystems, Foster City, CA) for verification of the obtained products.

The amount of specific anti-HIV-1 antibodies was proportional to the optical density (OD) values obtained by spectrometric analysis.

The matrix was sent to Aberdeen Proteomics for extraction, tryptic digestion, and mass spectrometric (MS) analysis using MALDI-TOF.

We independently confirmed these mass spectrometric observations by immunoblot analysis using anti-polyubiquitin antibodies that are linkage specific (Fig.  3B).

In addition, mass spectrometric analysis was used to assess the labelling ratio.