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The resulting solution was filtered, concentrated and pigment identity was confirmed by spectral scan using a UV VIS spectrophotometer between 400 and 700 nm wavelength.
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For a first impression of the changes occurring, spectral scans using diode array detection were performed.
Sequential spectral scanning uses tunable bandpass filters [17], [18], liquid-crystal filters [19] or acousto-optic filters [20].
The collected fVII-SnCe6 conjugate was characterised by spectral scanning (200 800 nm) and by reading absorbance at 635 nm (A635 nm) using a spectrophotometer (Beckman, Brea, CA, USA).
Data entry was by scanning using Cardiff Teleform.
To compare retinal thickness (RT) measurements between traditional 6 radial line scans and three-dimensional (3D) scans using spectral-domain optical coherence tomography (SD-OCT).
Spectral scans were obtained using a spectroradiometer (UNISPECTM, FieldSpec UV/NIR portable spectral system, PP Systems, Amesbury, USA) with the optic fibre, leaf-clip holder and reference provided by the manufacturer.
The spectral resolution was 4 cm−1 and 16 scans, spectral scanning conditions generally used, were performed.
The spectral sensitivities were measured with unpolarised light: we used a spectral scan method where a photoreceptor is clamped to a pre-selected DC potential by adjusting the light flux as we scanned from 300 to 730 nm in 10 nm steps with a monochromator.
Fluorescent microscopy (Zeiss AxioSkop 2 MAT) and confocal microscopy (Nikon 1AR Spectral Scanning Confocal Microscope) were used to confirm the presence of polymeric ssDNA product in the ODN-MSs generated by the RCA.
Systematic research on the deposited films thickness and optical properties was carried out by the methods of monochromatic (scanning) and spectral ellipsometry using the appropriate dispersion models.
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