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Herein we report a high-density genetic map for soybean, which is constructed based on large-scale markers developed by specific length amplified fragment sequencing (SLAF-seq).
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This process yields a size-dependent behavior characterized by specific lengths scales.
However, since ncRNA classes are usually not defined by a specific length but by a narrow distribution of lengths, it is reasonable to reward not only exact length matches but also small differences and to penalize large deviations of peaks in the length distributions.
We conclude that the basis for the lack of overt phenotypes in the Shortstop mice described by Slow et al. (9) is not explained by the specific length of the fragment.
However, we tested all dichotomies of short/long alleles and didn't observe significant differences, suggesting that increased and decreased expression of Fli1 may be modulated by a specific length of the GA microsatellite.
Recently, several cost effective methods of markers discovery and high-throughput genotyping were developed, such as RAD-seq (restriction site-associated sequencing), double digest RAD-seq, GBS (two-enzyme genotyping-by-sequencing), and SLAF-seq (specific length amplified fragment sequencing) [ 17, 18].
We describe a combination of electrochemical superfilling and oxidative cutting approaches to producing multilayer carbon nanocylinders (ML-CNCs) by cutting off a specific length of vertically-aligned MWNTs (VA-MWNTs) along their circumference.
The steel fiber contribution in the tensile zone after cracking needs to be considered in the structural design, and the compressive and tensile stress blocks in the cross-section should be considered based on the proposed material stress strain models (Fig. 16) by considering the equivalent specific length for tension.
Capital costs were calculated by combining the fracture specific length of stay with a daily capital cost rate [ 58] (inflated to 2009 [ 59]) (see Additional file 1: B.1.a).
The length of the rods can be cut to the specific length required by the surgeon by using a specially designed cutter.
This is implemented in the simulation model by the allocation of a specific length of stay in the hospital.
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