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The practical applicability of the immunosensor was demonstrated by specific detection of Sap secreted by the B. anthracis in culture broth just after 1 h of growth.
These goals are reachable with only small design modifications through simultaneous administration of multiple independent targeted phage particles in a single animal, followed by specific detection of each one with appropriate primers or probes in a multiplexed PCR.
The demarcation of epidermis and dermis layers was confirmed by specific detection of mRNA for E-cadherin (CDH1) and fibronectin (fn1) in the former and latter fractions, respectively (Figure 11B, middle and bottom panels).
Furthermore, according to OIE recommendations, suspected CBPP cases identified by serology should be confirmed by specific detection of the pathogen.
The 6-His-fusion constructs of hCNTF were verified by specific detection of the 6-His tag using Pierce 6XHis protein tag stain reagent set (Thermo Fisher Scientific Inc., USA).
Hence, combining high sensitivity and specificity, this assay appears to have excellent potential for SNP based disease diagnostics by specific detection of the low abundant mutated target in clinical laboratory settings.
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This conclusion is further supported by the specific detection of DOX in tumor tissues by HPLC.
This was demonstrated by the specific detection of Fusarium cerealis in grain samples of wheat.
Paraffin-embedded lung sections of BrdU-injected mice, controls (wild-type, empty-liposomes and siRNActrl/liposomes) as well as siRNAITSN-treated mice were incubated with anti-BrdU Ab, followed by CD31Ab, for specific detection of ECs followed by the appropriate fluorophore-conjugated reporters.
This was the case with the A/H1N1 (2009) strain, which was implemented in only a few weeks in the ResPlex II Plus Panel RUO [ 11], and, thus, the ResPlex III panel should also be updated rapidly by including the specific detection of the H1N1 (2009) virus.
All samples/isolates were also examined by PCR techniques for specific detection of Acanthamoeba DNA and to determine genotypes of the particular strains.
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