These discrepancies may be resolved in future experiments either by standardising RNA levels to the expression of cell-specific markers (such as CD4) to correct for the difference in cell number or by analysing specific cell populations by flow cytometry rather than by qPCR.
By targeting specific cell populations and modifying the genes delivered, models of medulloblastoma, brainstem glioma, astrocytoma, oligodendroglioma, and GBM have been described (Table 1).
It can be addressed in a high throughput manner by isolating specific cell populations using laser microdissection from tissue sections (laser capture), purification of RNA and quantitative RT-PCR gene expression profiling, comparing control and mutant tissue from,, for example the hypertrophic, prehypertrophic or the elbow joint region alone.
In conclusion, the data support the hypothesis that chronic exposure to HIV-1 Tat may play a significant role in the decline of cognitive and motor function in HIV patients, perhaps related to or driven by changes in specific cell populations.
The experimental design for this analysis was to transduce primary MECs overnight with the EF1α- PyMT-ZsGreen lentivirus [ 12], followed by FACS enrichment of specific cell populations and transplantation with an excess of uninfected and unsorted MECs (Fig. 1a).
We tested differential expression in specific cell populations by using the same variable selection strategy but allowing for an additional single interaction regressor (in addition to the 4 reference signals).
The winning recipe for tissue regeneration is likely to be found ultimately by identifying the genetic elements and specific cell populations that limit or allow intrinsic potential.
While it is well-established that endogenous progenitors can be activated toward bone formation by overdoses of single morphogens, the challenge to stimulate the healing processes by coordinated and controlled stimulation of specific cell populations remains open.
Our approach to defining cell surface signatures in differentiating cultures of pluripotent human stem cells will potentially enable quantitative benchmarks to be devised for comparing specific cell populations generated by different hESC and hiPSC lines.
