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Four or five selection rounds on the tumor cells were necessary for mixLIB and scFvB96 libraries, respectively, in order to obtain phage pools enriched by specific cell binders (Fig. 3a).
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Using a human T cell line, gene-modified to express a tumor-specific CIR on their surface, and a human tumor cell line we demonstrated that combining CIR-mediated activation with FACS sorting of CD69+ T cells, it is possible to isolate binders to tumor specific cell surface antigen, with an enrichment factor of 103-fold after two rounds.
Based on an effective interaction between the CIR and the tumor antigen, we demonstrated that combining CIR-mediated activation with FACS sorting of CD69+ T cells, it is possible to isolate binders to tumor specific cell surface antigen, with an enrichment factor of at least 103-fold after two rounds, resulting in a homogeneous population of T cells expressing tumor-specific CIRs.
Specific cell surface receptors for plasminogen (Pg) are expressed by a wide variety of cell types.
Viruses favor specific cells.
Enough amount of scFv-Fc could be obtained from transient expression by using the resulting constructs in HEK293E cells, which enabled rapid identification and characterization of specific binders to cell surface CD9 using flow cytometry, immunoprecipitation and immunofluorescence confocal microscopy.
The use of tethered RNAs to detect RNA protein interactions was described by Lioka et al. [ 79], revealing specific protein binders.
In this paper, we screened scFv "phage display" library on suspension cultures of HEK-293 cells expressing CLDN1 on plasma membrane, from which we successfully isolated specific CLDN1 binders.
Dissecting complex protein structures requires highly specific affinity binders.
IL-17 and RORγt expression by MeV-specific T cells.
Thus, a competitive selection could be imposed by saturating the non-specific binders, M13KO7 with the competitive proteins (lysate and PTM) thereby promoting binding of αUbi towards purified rUbi32.
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