Sentence examples for by specific amplification of from inspiring English sources
Mouse Xist RNA probes were generated by specific amplification of exons 1a and 6.
The analysis was performed by specific amplification of region of interest by PCR and followed by multi-temperature single-strand conformation polymorphism (PCR-MSSCP) technique.
The fusion gene, inserted into the plasmid vector pHBM905B, was confirmed by DNA sequencing, and the positive transformants (P. pastoris GS115 (his4)) were identified by specific amplification of the fusion gene (PCR) using genomic DNA as templates.
The presence of Nanoarchaeota was confirmed by specific amplification of the SSU rRNA gene with the primers 7mcF and 1511 mcR [ 7] and Sanger sequencing and by 454 pyrosequencing of the V4 region of the SSU rRNA (9003 total archaeal sequences) as previously described [ 9].
All 99 A. flavus isolates included in this study were identified as belonging to Aspergillus section Flavi by specific amplification of a DNA fragment of expected size (~243 bp) in PCR with Aspergillus section Flavi-specific primers AFLF and AFLR [ 10].
To confirm that FVIII was synthesised in endothelial cells, polyadenylated FVIII transcripts were analysed by reverse transcription (rt) using an oligo-dT primer and amplification of cDNA products by specific polymerase chain reactions (PCR).
The CYP21A2 gene dosage assessment by Real-Time PCR and by the specific amplification of each duplicated gene showed that 8 out of the 144 individuals were carriers of CYP21A2 gene duplication with one of the copies p.Gln318X mutated (UniProtKB ID P08686).
The existence of an additional duplicate, AgAcp34A-3, was then confirmed by obtaining specific amplification of both paralogs in all individuals and by observing for each gene duplicate paralog-specific heterozygous SNPs (double peaks) in chromatograms.
ChIP analysis of UF5 cells demonstrated that EWS/WT1 −KTS) was present near the GC-rich region of ENT4 promoter as shown by the specific amplification of the GC-rich sequences with the chromatin immunoprecipitated with C-19 antibodies but not with the IgG-immunoprecipitated chromatin control (Fig. 3B, UF5 panel).
CCHFV RNA was detected by the specific amplification of a fragment of the viral S-segment using the protocol of Tino et al., 1996 [ 15].
Mutations were screened in the proband of the family by locus specific amplification of PKD1 and direct sequencing of exonic and flanking intronic regions of PKD1 and PKD2 [ 4].
