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The proteins were released by sonication of a silk mat layer in normal saline.
Experimental parameters influencing the LLUSAE were optimized using an univariate design, resulting in a 30-min ACN/MeOH extraction by sonication of a lyophilized sample, followed by PSA/C18/MgSO4 clean-up and fast centrifugation before UPLC analysis.
The vesicles were prepared by sonication of a lipid solution containing DPhPC and cholesterol.
Emulsion particles were prepared by sonication of a triolein/egg yolk PC mixture (3.5 1, w/w) in TBS (pH 7.4).
Emulsion particles (86 ± 7 nm diameter) were prepared by sonication of a triolein/egg yolk PC mixture (3.5/1w/w) in pH 7.4 Tris buffer.
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To make α-TEA soluble in aqueous culture media for in vitro assays, α-TEA was vesiculated (Vα-TEA) by sonication of an α-TEA thin film in the presence of PBS as previously described [ 7].
There was no statistically significant difference in the number of S. epidermidis colonies recovered by sonication of steel discs (method A) and scraping followed by sonication of the surgical blade (method D) (p = 0.4; Figure 3).
Protein extracts were prepared by sonication of tissues in a 0.25 M sucrose solution.
The formation of Ga-Pt particles and their simultaneous embedment in graphene layers was performed by sonication of molten gallium in an aqueous solution of H2PtCl6 in the presence of graphene.
Chromatin was sheared to 400 600 base pair fragments by sonication of the cell lysate using a twin Bioruptor (Diagenode, Belgium) for 15 cycles, 30 s on, 30 s off on high setting.
Fe3O4 can be simply synthesized by sonication of iron II)acetate in water under an argon atmosphere.
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