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This was confirmed by solution analyses with UV Vis [62, 63] which showed that dissolved HA concentrations were below the detection limit of < 2 mg L−1, consistent with > 98% removal of dissolved HA during coating.
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Nodal support was calculated by bootstrap analyses with 1000 replications.
Samples were left to extract for 7 days at ambient temperature and the resulting solutions analysed by gas chromatography (GC) with flame ionization detection by a previously described method (Keefover-Ring 2013).
The closed form analytical solutions derived were verified by performing strain energy release rate analyses with taking into account the material non-linearity.
The obvious general solution is to report all subgroup analyses with equal prominence.
We executed MP analyses with the branch and bound search option, which guarantees an exact solution.
The extract solution was analysed by direct infusion with flow rate of 10 µL/min.
Cells were washed, fixed with 2% paraformaldehyde solution and analysed by flow cytometry using a CyAn ADP flow cytometer (Beckman-Coulter).
The cells were rinsed before being resuspended with fluorescein-PRB-1 antibody solution and analysed by flow cytometry in the presence of propidium iodide/RNase solution.
The reaction products in solution were analysed by ionic chromatography.
Protein aggregation behaviour in solution was analysed by dynamic light scattering (DLS) using a DLS802 system from Viscotek (Viscotek Corp., Malvern Instruments, Malvern, Worcestershire, UK).
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