The MeJA induction time and feeding concentration, known to be crucial to plant cell culture, were investigated by single-factor experimental design by varying a single factor at a time and keeping other factors at a constant value.
A 4-way single factor experimental design was used to test effects of these influences on handler beliefs.
But this study was based on single-factor experimental design, and did not systematically optimize the ATPS process.
Response surface methodology (RSM), which includes experimental design, model fitting, validation and condition optimization, has eliminated the drawbacks of single-factor experimental design and been proved to be powerful and useful for the optimization of ATPS [14], [15].
The experimental data obtained in bonding tests were analyzed by single factor ANOVA variance type, followed by post hoc Fisher's LSD type, with a significance level of 5%[14]4].
The extraction conditions were firstly optimized by single factor experiments.
The strategies were driven by the development of a four single-factor experimental design and were based on the change of culture media composition in terms of carbon and nitrogen and the modification of the pH control by using NaOH or NH4OH.
From the single-factor experimental design, extraction with hexane resulted in the highest yield compared to petroleum ether and isopropanol.
For this, the effects of the sprouting conditions (temperature, time and pH of stress medium) on the hydroxyl radical inhibition were investigated carefully by single-factor experiments and statistical experimental designs, which included a fractional factorial design, steepest ascent experiments, a central composite experimental design and response surface methodology.
The influential parameters of NADES-MAE were investigated and optimized by single-factor experiment and Box Behnken experimental design combined with response surface methodology.
The extraction procedure was optimized systematically by single-factor experiments and a Box-Behnken experimental design combined with response surface methodology.
