Sentence examples for by single cell sorting from inspiring English sources

These cells were automatically collected by single cell sorting into individual wells of 384-well plates.

Of 15 Tg+ B cells isolated by single cell sorting, 14 expressed a γ2b heavy chain; none expressed a μ heavy chain (data not shown).

Lentiviral transduced cells were selected by growth in puromycin, clones were isolated by single cell sorting, and characterized by anti-FAK immunoblotting as described [ 17].

The efficiency of the procedure was determined by spiking blood with SKBR-3 cells, enrichment with the CellSearch system, followed by single cell sorting by fluorescence-activated cell sorting (FACS) and whole genome amplification.

We confirmed that RSC colonies were derived only from pigmented cells by single cell sorting: 1.8% of single pigmented cells gave rise to clonal colonies and none arose from the non-pigmented cell fraction (n = 1691 cells, 2 experiments).

We simultaneously constructed corresponding combinatorial cDNA antibody Fab libraries except that construction of a cDNA Fab library using pt1 PBMCs was omitted because the isolation of bnmAbs by single cell sorting of memory B-cells from pt1 PBMCs has been reported [18,28].

The sort criteria were optimized for a Lysotracker region that contained 5 10% heterotrophic Paulinella by positive microscopic identification, prior to single cell sorting.

The cell pool was subsequently single cell sorted by FACS and clones analysed for RAB8A depletion by immunoblotting and sequencing.

(A ) Left, touch aligned spike rasters for a single cell, sorted by one of three touch properties: Order of touch in trial (top), whisker velocity at touch onset (middle), maximum whisker curvature during touch (bottom).

One chosen T-cell clone was retrovirally transduced with a second TCR and single cell sorted for the TCRvβ chain expressed by the LTCRSN vector.

Molecular analyses showed that parasite isolates obtained by single-cell sorting were highly homogenous.