The introduction of PTEN to BRCA1 mutation-prediction algorithms is supported by single-cell analyses of temporal somatic events in BRCA1 breast tumor tissue, which revealed that loss of PTEN is an early event in the development of BRCA1 basal-like tumors, whereas TP53 mutations occur first in most luminal BRCA1 tumors [ 12].
Single cell 'omics' requires a technological platform with reliable and high throughput single cell analyses with single molecular detection and quantification.
Remarkably, while cytokines appear coordinated in cell population assays ('pro-inflammatory cytokines' induced by highly similar cell signaling pathways), our single cell analyses reveal that the level of production of one cytokine in a cell is not predictive of the level of production of other cytokines within the same cell.
Single cell analyses were carried out on day 7 of culture by intracellular staining and flow cytometry using fluorescent monoclonal antibodies to CCL4 and p24 as markers for β-chemokine and HIV-1 infection, respectively.
Single cell analyses revealed mutational heterogeneity among CTCs and tumor cells in tissues.
This process indeed runs a significant risk of contamination of both the endogenous PCR product by exogenous DNA, and of the laboratory dedicated to single-cell analyses by the endogenous PCR product.
The DNA damage was analysed by single cell gel electrophoresis (comet assay).
This electromagnetic needle may be useful in single-cell analyses because magnetic particles can be injected into the cell by magnetic force, without requiring specific functionalization to bind particles to the needle body.
To validate these findings, we analysed individual E6.25 epiblast cells by single cell real-time quantitative polymerase chain reaction (RT-qPCR).
The model predicted distributions of single cell responses that were confirmed by single cell mRNA measurements.
We were also concerned about off-pathway effects of nocodazole treatment, which prompted us to assess both Hsp104 expression and localization in the presence of this treatment, and to further test the suggestions raised by these arrest experiments using single-cell analyses in asynchronous cultures.
