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Genotyping was by simple sequence analysis on agarose gel, as described previously [ 43- 45].
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Since contamination of DNA from out-crossing plants critically impacts on estimation of mutation density and identification of mutant, out-crossing plants was carefully examined by simple sequence repeat (SSR) analysis.
All genotyping was by simple sequence length polymorphism (SSLP).
In contrast, the differences between PriA and subHisA sub-families, which show less sequence divergence despite a big functional leap, facilitate the analysis of this process by simpler sequence comparisons (LNG & FBG, unpublished results; [ 27]).
Putative PttMAP20 orthologs were easy to detect among monocots and dicots by simple analysis of sequence similarity.
In both bovine59 and human aggrecan15, the amino acid sequence of this region, revealed by cDNA sequence analysis, contains a striking repeating sequence.
In fact, there are many groupings of bacteria that are not supported by RNA sequence analysis.
Mutations were verified by DNA sequence analysis of the resulting clones.
Mutants were confirmed by DNA sequence analysis.
Analysis was done by Chromas Sequence Analysis software.
EST sequencing has also been used to establish phylogenetic relationships and identify simple sequence repeat (SSR) analysis.
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