Twenty-two samples were positive for HCoV-NL63 by both simple and multiplex RT-PCR.
The comparative analytical sensitivities of these simple and multiplex RT-PCR assays were previously studied on prototype strains by analyzing serial 10-fold dilutions of positive control for HCoVs NL63, OC43, and 229E.
A multiplexed bioassay platform was constructed for simple, sensitive, and multiplex analysis of target DNA sequences.
A disposable, paper-based PCR-dipstick DNA chromatography method was developed for simple, rapid and multiplex analysis of bacterial profiles in microbiota by impregnating several oligonucleotides on one strip to specifically recognize target DNA sequences.
Since concentration and size distribution (as assessed by ethidium bromide agarose gel electrophoresis) of genomic DNA isolated from FFPE tissue are inadequate predictors per se for aCGH success, we have developed a method for DNA isolation from FFPE tissue with a subsequent simple and reliable multiplex PCR protocol that predicted successful aCGH with high accuracy.
This study describes a simple and rapid multiplex-PCR method to determine the ability to produce histamine, tyramine and putrescine by bacteria.
However, the examination of LGR prior to the laborious exon-by exon mutation scanning of the MMR genes has also been suggested and is feasible by applying simple and robust techniques, such as MLPA (multiplex ligation-probe dependent amplification) [ 18].
These mutations are not usually detected by conventional methods of mutation analysis, such as denaturing high-performance liquid chromatography (DHPLC) and direct DNA sequencing, but they are detectable by a simple and robust technique such as the Multiplex Ligation-Probe Dependent Amplification (MLPA) [ 18, 19] assay.
Blood cells and serum cytokines were analyzed by CBC analysis and multiplex ELISA.
The method was a simple, fast, cost-effective and multiplex sequencing library approach.
