Sentence examples for by silver staining using from inspiring English sources

Samples were run on SDS-polyacrylamide gels and analyzed by silver staining using GelCode SilverSNAP (Pierce, Rockford, IL) kit, as described by the manufacturer.

Pull down eluates were heat denatured in Laemmli sample buffer and fractionated on 12% SDS-polyacrylamide gels, fixed and visualized by silver staining using standard protocol.

Five microliters of the concentrated fraction were loaded onto 15% acrylamide gels and were verified for purification by silver staining using the BioRad silver staining kit (BioRad).

After electrophoresis, protein bands on the gel sheets were visualized by silver staining using the procedure described by Mortz et al. [ 11].

DNA fragments were visualised by silver staining using the Plus One DNA silver-staining kit (Pharmacia Biotech, Tokyo, Japan) following the manufacturer's manual.

The reaction is resolved on 4%–12% SDS-PAGE gradient gels run in MES buffer (Invitrogen) and stained by silver staining using the SilverStain Plus kit (BioRad).

Following photo imaging of chitinase activity banding patterns, the agarose layer was gently removed from gel and gels were rinsed in deionized water and stained for protein profiles by coomassie or silver staining using standard procedures.

Following heat denaturation for 3 min, the proteins bound to the roscovitine matrix were separated by 10% SDS-PAGE followed by immunoblotting analysis or silver staining using a GE Healthcare SDS-PAGE silver staining kit.

Following heat denaturation for 3 min at 95°C, the proteins bound to 6AP or GA matrices were separated by 10% SDS-PAGE (precast NuPAGE -Invitrogen- 1 mm thick gel) followed by immunoblotting analysis or silver staining using an Amersham SDS-PAGE silver staining kit.

Proteins were separated by SDS-PAGE and silver stained using SilverQuest (Invitrogen) following manufactures instructions.

Gels were silver stained using a protocol suggesting by Blum et al. [ 58] and repeated in triplicate.