Sentence examples for by silencing its expression from inspiring English sources

The interactions between NGF and the other neurotrophic factors and receptors during rgRSV infection were investigated by silencing its expression in epithelial cells with a specific siRNA (Dharmacon, Chicago, IL).

The results we describe herein provide new insights for applying STMN-1 as a potential target for antitumor therapy by silencing its expression with STMN-1 shRNA.

Therefore, we used the SNU-478 cells to investigate the tumor promoting role of AGR2 by silencing its expression with an AGR2 shRNA.

To establish causality between the induction of activin A in epithelial cells and the production of desmoplasia-associated proteins in reprogrammed HMFs, we determined whether activin A was necessary for the induction of these genes by silencing its expression in vHMECs with a short-hairpin RNA to activin A (sh Activin A-vHMEC), as described [ 5].

Synthetic siRNAs appeared as an important research tool for understanding the function of a gene by silencing its expression when Elbashir et al (2001a) reported that gene expression in mammalian cell lines can be effectively silenced by transfecting cells with synthetic siRNAs.

This study represented an interesting proof-of-principle, but it was limited in that the inferred gene network was not genome-wide but included only a few selected genes; in addition, the method required each of the gene in the network to be perturbed, by either silencing its expression, or by inducing it, thus making the scaling up of the approach to the genome level very difficult.

Blocking IKKβ activity with sodium salicylate, a potent inhibitor of IKKβ [30], or silencing its expression by shRNA also lowers the etoposide-induced activation of caspases (Figs. 5B and 5C, columns 4 and 8, respectively).

In order to investigate if the loss of CCDC6 could affect hydrogen peroxide-induced apoptosis in CCDC6-expressing primordial GC1 cells, we silenced its expression by the transient transfection of specific shRNAs directed against murine CCDC6 and exposed the cells to different doses of hydrogen peroxide for 1 hour.

Transfection of CaSki cells with sieIF2α efficiently silenced its expression by ∼80%; siB5 had little or no effect on eIF2α expression (Fig. 9A).

As Impβ has been shown to function as a nuclear import receptor for proteins containing a conventional bipartite NLS, we tested its involvement in MRTF-A nuclear import by use of siRNA to silence its expression.

The lncRNA Airn is transcribed in antisense orientation to the maternally expressed Igf2r gene and controls Igf2r mRNA expression by silencing its transcription in cis.