Clade II is the second largest cluster supported by significant bootstrap values and consists of five sequences (At2g39060, At3g07950, LOC_Os03g24390, LOC_Os04g01300 and LOC_Os07g46170).
Phylogenetic analysis revealed that RPS27-1 and RPS27-2 sequences grouped in two separate clades supported by significant bootstrap values.
NSP1, NSP4, and NSP5 clustered with sequences from artiodactyl hosts, however branching was not supported by significant bootstrap values.
We annotated the Artemia unigenes by comparing to protein sequences collected from public databases, and 3,953 unigenes (49.3%) showed significant sequence similarities (e-value < 1e-5) to known genes.
* Contigs that shown significant sequence homology (e value > 10-10) with contigs from others hierarchical clusters.
Additionally, both for initial candidate identification and for validation by reverse BLAST, sequences were analysed by alignment for the presence of significant sequence similarities, and also were parsed through NCBI CDDB rather than relying solely on e-value cut-offs.
Significant sequence similarity was defined as an expect (E) value <1×10−4.
A very low E-value (generally <0.001) generally indicates a highly significant sequence match.
Of the 5,126 sequences, 1,420 sequences were found to be likely of plant origin as indicated by significant BLAST scores (E value ≤ 10-5) to plant sequences but little or no homology to other organisms.
Of these, 11 have significant sequence homology.
