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Experiments were carried out by shaking the samples by means of the laboratory shaker type 358A (Elpin Plus, Poland).
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Epithelial cells were released by shaking the tissue samples for 5 min at 700 rpm using an orbital shaker after which the remaining tissue was removed from the tube and fixed in methacarn for analysis of isolation efficiency.
Samples were mixed by shaking the plate in the plate reader for 10 s before it was incubated for 1 h at 37 °C.
The crushed samples were then extracted with 25 mL of double deionised water by shaking the suspension continuously on orbital shaker for an hour.
Samples were extracted with hexane:methyl tert-butyl ether (1 1, vol/vol) three times by shaking the tube > 5 min each time.
Homogenize the solution by shaking the phantom.
Mating was interrupted by shaking the vial containing the pair.
Conidia were harvested by shaking the leaves in sterile water.
For seed germination test, water was extracted from the samples by shaking fresh samples with double distilled water (DDW) at solids: DDW = 1 10 (w/v) for 1 h, then suspensions were centrifuged at 3,000 rpm for 20 min before filtering through Whatman No-1 filter paper.
Nitrate was extracted by shaking soil samples (20 g dm) with 0.1 M KCl (100 mL minus the water content of the soil sample) for 60 min at 150 rpm.
Air bubbles were then removed from each tube by shaking, and samples were centrifuged at 14,000 rpm for 20 minutes at 20°C.
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