MSCs were released from PRP-SF bioscaffolds by shaking the sample with 400 µL Urokinase VEDIM 250000 UI/vial (UCB Pharma S.A) for 3 hours at 37 °C in a 5% incubator.
Air bubbles were then removed from each tube by shaking, and samples were centrifuged at 14,000 rpm for 20 minutes at 20°C.
Its two major characteristics are: (1) if gentle mechanical stress is imposed onto G⊃ACN in the gel state (for instance, by shaking sample vial), this causes the fibrous gel scaffolding to break down to yield a sol; and (2) more vigorous mechanical agitation of the reactant mixture ultimately produces only precipitation, prohibiting any further gelation from occurring.
Experiments were carried out by shaking the samples by means of the laboratory shaker type 358A (Elpin Plus, Poland).
Nitrate was extracted by shaking soil samples (20 g dm) with 0.1 M KCl (100 mL minus the water content of the soil sample) for 60 min at 150 rpm.
For seed germination test, water was extracted from the samples by shaking fresh samples with double distilled water (DDW) at solids: DDW = 1 10 (w/v) for 1 h, then suspensions were centrifuged at 3,000 rpm for 20 min before filtering through Whatman No-1 filter paper.
Nitrate was extracted from soil by shaking samples (20 g dm) with 0.1 M KCl solution at a ratio of 5 mL of KCl solution per gram dry weight for 60 min at 150 rpm.
Epithelial cells were released by shaking the tissue samples for 5 min at 700 rpm using an orbital shaker after which the remaining tissue was removed from the tube and fixed in methacarn for analysis of isolation efficiency.
Nitrate-N and NH4-N were extracted from soil and manure by shaking 2 g of sample in 2 M KCl solutions for 1 h in 25 mL polypropylene centrifuge tubes on an end-over-end shaker, then centrifuged at 10,000 rpm for 10 min and supernatant was analyzed.
Calcium and magnesium were analyzed by shaking 1 g of compost sample with sodium acetate solution for 1 h prior to atomic absorption spectroscopy detection (Varian Spectra AA 220FS, USA).
