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The project had been affected by several changes of policy, including government vacillation over the increasing costs of a building intended mainly for scholars and totaling £4500 million.
Gels were stained with Coomassie blue for 1 hour and destained in 40% methanol/7% acetic acid for 1 hour, followed by several changes of 5% methanol/7% acetic acid.
Tissues were cleared by several changes of 96% ethanol and stored in 75% ethanol.
To remove the chlorophyll, leaves were placed into 95%% ethanol and boiled in a water bath, followed by several changes of the solution.
The DAB-treated leaves were removed, placed into acetic acid/glycerol/ethanol (1 1 1, vol/vol/vol), and boiled for 5 min in a water bath, followed by several changes of the solution.
After a pre-treatment with 10 ng/ml mitomycin C (Carl Roth GmbH, Karlsruhe, Germany) for 45 min to inhibit proliferation, a scratch was set with a pipette tip in the cell monolayer, and the floating cells were washed away by several changes of media.
Similar(54)
To sum up, the development of transport policy programs over the last 20 years clearly shows varying priorities caused by several changes in the composition of the federal government.
These developments might partly be explained by several changes in the working life of GPs.
Exercise is accompanied by several changes in the morphology and physiology of different organs and tissues.
Tl+-bound GltPh-R397A crystals were obtained by soaking GltPh-R397A apo crystals in several changes of solutions containing 18 20% PEG 400, 0.1 M citric acid/Tris pH 4.5, 0.1 M TlNO3, 7 mM n-decyl-α-D-maltopyranoside and 10% glycerol.
Made several changes of costume.
More suggestions(16)
by repeated changes of
by several gallons of
by regulated changes of
by several shouts of
by subtle changes of
by frequent changes of
by weekly changes of
by minor changes of
by sclerotic changes of
by several members of
by topographic changes of
by small changes of
by several decades of
by rapid changes of
by several aspects of
by several factors of
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com