Sentence examples for by serial passaging through from inspiring English sources

Cells were further dissociated by serial passaging through a syringe with 25-gauge needles.

The strain of HCMV used in those studies was the Towne strain, which has been attenuated by serial passage through human fibroblast cells [3].

Cells were filtered through a 40  μm nylon mesh and were further dissociated by serial passage through serological pipettes.

We have previously characterized this strain and maintained it by serial passage through jirds (Meriones unguiculatus) and ticks.

Tumour tissues were maintained by serial passage through Non Obese Diabetic Severe Combined Immunodeficient Mice (NOD-SCID) mice [26].

The cells were disrupted by serial passage through a 26 gauge needle and stored at -80°C before use.

Cells were passaged through a 40 μm mesh screen, and were further dissociated by serial passage through a syringe with 18 to 25 gauge needles.

The RCA product was sheared by serial passage through a narrow aperture using a Hydroshear device (Gene Machines), and the resulting fragments were enzymatically repaired to blunt ends and gel purified, then ligated into pUC18 plasmids.

A previous report found that a duck-derived H9N2 virus required experimental adaptation by serial passage through quail and chicken lungs in order to replicate efficiently in those species.

Brain tissue homogenates (10% w/v) from frozen samples of frontal cortex were prepared in Dulbecco's phosphate buffered saline lacking Ca2+ or Mg2+ ions using tissue grinders or by serial passage through needles of decreasing diameter.

By serial passage through mice i.e. recovery and reculturing of a M4A4 lung metastasis, we have previously selected a subclone which is more aggressively metastatic than its parental M4A4 cell line.