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In brief, all DNA, including the C3 plasmid within the cells, was collected by serial extraction with phenol/chloroform and ethanol precipitation as usual and was treated with bisulfite sodium.
Similar(59)
We hypotonically extracted soluble proteins from purified schizonts or merozoites, followed by serial extraction of the insoluble pellet with a high-salt buffer and sodium carbonate buffer, pH 11.0 to separate peripheral from integral membrane proteins.
Using serial extraction with hexane, CH2Cl2, and MeOH, Motta et al. [ 20] identified a great diversity of diterpenes, especially in the CH2Cl2 extract.
The dry pellet was reconstituted in 300 μL of water, and lipids were removed by serial liquid liquid extraction with 500 and 300 μL of chloroform, respectively.
Lipids were isolated by serial liquid/liquid extractions with ethyl acetate.
Similar to serial extraction, the methanol solvent extracted the highest level of phenolics).
A recent study showed that serial extraction of sFlt-1 from the plasma of severe preeclamptic patients by apheresis reduced circulating sFlt-1 and stabilized maternal blood pressure, prolonging gestation in preeclamptic women [ 41].
Exact quantification was achieved by serial dilution with cDNA produced from total RNA extracts using serial dilution steps.
Lanes 1 5: serial extractions from polished seed; lanes 6 7: serial extractions from polishing waste.
The paper discs were impregnated with AgNPs solutions that were obtained by serial dilution with distilled water (150 µg ml−1).
Between the serial extractions, the funnels were vigorously shaken for 2 min.
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