Sentence examples for by sequential washes with from inspiring English sources

Bacteria were made electrocompetent by sequential washes with ice-cold sterile 10% glycerol, and transformed with approximately 500 ng of each purified PCR product.

This was followed by sequential washes with low salt wash buffer (1% Triton X-100, 0.1% sodium deoxycholate, 50 mM Tris pH 8.0, 150 mM NaCl and 5 mM EDTA), high salt wash buffer (1% Triton X-100, 0.1% sodium deoxycholate, 50 mM Tris pH 8.1, 500 mM NaCl and 5 mM EDTA) and LiCl wash buffer (0.25 M LiCl, 0.5% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA and 10 mM Tris pH 8.1).

Proteins retained on the column were displaced by sequential washes with cysteine, glutathione, and dimercaptopropanesulfonate (DMPS) after removing nonspecific binding materials with buffer and ethylene glycol.

Overnight hybridization was followed by sequential washes with wash buffer 1 (50% formamide, 5× SSC, pH 4.5 and 1% SDS in PBT) followed by buffer 2 (50% formamide, 2× SSC, pH 4.5, and 0.1%Tween-20Tween-20) at 63°C to remove unbound probe.

The absorbent was pre-conditioned by sequential washing with 10 mL of methanol and 10 mL of 0.05 M sodium dihydrogen phosphate solution.

The final product was collected via centrifugation after reaction for 1 h, followed by sequential washing with distilled water three times and ethanol one time and then air drying at 50 °C for 15 h.

Briefly, after rehydration by sequential washing with gradient dilution of MeOH, the embryos were heated with 150 mM Tris-HCl at pH 9.0 as in the immunostaining.

Spores were isolated by sequential washing with dH2O, TBS-Tween 20 (0.3%), and TBS, followed by Percoll™ centrifugation, a final wash with TBS-SDS (0.1%), and genomic DNA was extracted using established protocols [78].

Proteins binding nonspecifically were removed by sequential washing with a prepared washing solution (4% DMSO in PBS) followed by elution of the proteins in the loading buffer and denaturation by heating (70 °C, 10 min).

Briefly, 4 × 10 cells were treated with nocodazole (10 μg/ml) and cytochalasin B (5 μg/ml) for 60 min, followed by sequential wash with cold PBS, 8% (w/w) sucrose in 0.1 × PBS, and 10 mM Tris HCl, pH 8.0.

After rehydration by sequential washes in 75, 50 and 25% ethanol followed by four washes with phosphate-buffered saline (PBS), the fixed and rehydrated cells were treated with 10% goat serum in PBS and then exposed to the primary antibody (rabbit polyclonal anti-reovirus type 3 serum diluted 1/5000 in PBS) overnight at 4°C.