Sentence examples for by sequential transduction with from inspiring English sources

Along these lines, primary pig fibroblasts [ 44] and human breast epithelial cells [ 51] were transformed by sequential transduction with dominant negative p53, activated CYCLIN-dependent kinase complex (Cyclin D1/CDK4), c-Myc, H-Ras and telomerase.

Compared with culture in free-floating aggregates, exocrine cells in 2D lost their Pdx1 expression, but by sequential transduction with LeMAPK and LeSTAT3, the number of Pdx1+ cells in 2D cultures increased again.

We recently developed an efficient hepatic differentiation method from hESCs and hiPSCs by sequential transduction of FOXA2 and HNF1α.

EBOECs and GBOECs were successfully produced by retroviral gene transfer by sequential transduction and sorting by FACS.

To the best of our knowledge, this is the first report on the transformation of feline ECs by sequential lentiviral transduction with SV40LT and hTERT genes.

J16 cell lines with stable, Dox-inducible expression of BH3-only proteins were created by sequential lentiviral transduction with pLVX-Tet-On Advanced (Clontech) and pLVX-tight encoding the BH3-only proteins.

Mixed pools of MEF-BAK−/− cells that co-expressed proteins with FKBP or FRB moieties were generated by sequential retroviral transduction.

Protein extracts from subclones of iGFP-WT and iGFP-NLSm cell lines were obtained by sequential extraction with RIPA followed by urea buffer and analyzed by immunoblot.

Double-labeling immunofluorescence was performed by sequential incubation with the appropriate primary antibodies.

Immunoreactivity was detected by sequential incubation with horseradish peroxidase-conjugated secondary antibody (Santa Cruz).

This was then followed by sequential incubations with blocking solutions containing primary and secondary antibodies.