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iPSCs are specified to primitive streak/mesendoderm/definitive endoderm by sequential stimulation with liver development-related cytokines, resulting in differentiated cells with characteristics of HLCs.
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This underestimation can be reduced by sequential stimulation and co-culture of patient and donors cells to activate a greater fraction of proviruses (Ho et al., 2013), but this approach is impractical because the VOA, even with a single round of stimulation, requires a large blood volume, weeks to complete, and costs more than $1000 per assay for reagents and labor.
(3) Sequential stimulation with LD first followed by HD (LD+HD) can activate x3 to a maximum level that is at least 50% higher than that under HD alone.
Sequential stimulation of the cultures with a proinflammatory cytokine (IL-6) and then LPS decreased MTT reduction by about 50%.
The force frequency relationship was measured by sequential supramaximal stimulation for 1 s at 10, 30, 50, 100, and 150 Hz, with 2 min between each stimulation train.
The force-frequency relation was determined by sequential supramaximal stimulation for 1 second at 5, 10, 20, 30, 50, 100, 120, and 150 Hz, with 2 minutes between each stimulation train.
Thus, sequential stimulation of Bhlhb2, Ngn3, and NeuroD expression, paralleled by reduced Hes1 expression, precedes RA-stimulated differentiation of β-cells.
Speech recognition scores were correlated with measured electrical-field interaction for the strategy which used simultaneous stimulation but not the strategy which used sequential stimulation.
Correct identification of vowels, consonants, and words in sentences was measured with two speech strategies: one which used simultaneous stimulation and another which used sequential stimulation.
To induce LTP or LTD, sequential stimulation (SSt) that consisted of 600 pulses at 5 Hz, or at 1 and 10 Hz was delivered to ipsilateral Schaffer and contralateral commissural pathways with stimulation timing between both inputs indicated by the inter-peak intervals (Δt) of the fEPSPs (see inset, Fig. 2).
We termed such stimulation over multiple active electrodes as spatially distributed sequential stimulation (SDSS).
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