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A series of branched and folic acid (FA) conjugated amphiphilic block copolymers were synthesized by sequential steps of ring opening and atom transfer radical polymerizations.
Briefly, neurons were obtained by sequential steps of enzymatic digestion and mechanical dissociation of DRGs harvested from P0 animals.
Briefly, after preparation of the system by sequential steps of energy minimization and equilibration, the production phase was carried out at constant temperature (310 K) and pressure (1 atm) conditions.
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The catalytic cysteine thiol is first oxidized to the sulfenic (-SOH) form, which is amenable to reduction by cellular enzymatic mechanisms or with reducing agents in vitro, followed by further sequential steps of oxidation, to sulfinic (-SO2H) and sulfonic (-SO3H) forms, leading to irreversible PTPase inactivation.
BACs were modified by two sequential steps of retrofitting using the lambda recombination system [ 63].
Finally, early steps in the maturation process of microvascular networks for 14 d were characterized by demonstrating sequential steps of branching, expanding, remodeling, pruning, and clear delineation of lumens within fibrin gel scaffolds.
In our experimental results, vascular maturation is characterized by the sequential steps of branching, expansion, remodeling, pruning, and lumen formation in the microvascular networks within the FGSs (Fig. 5).
Liver development proceeds by sequential steps during which gene regulatory networks (GRNs) determine differentiation and maturation of hepatic cells.
The PCR program consisted of activation at 95°C for 15 minutes followed by cycling with sequential steps of denaturation at 95°C for 15 seconds, annealing at 58°C for 30 seconds and extension at 72°C for 15 seconds with the last step being used for data acquisition.
Once the first nucleobase was installed on the solid support, the ON growth was obtained by repeating the following sequential steps of the automated ON synthesis: Figure 1 Synthetic procedure for solid-phase synthesis of aminosilane-modified mesoporous silicon.
The functional consequence of differences in anatomical architecture was tested by constructing network models representing sequential steps of mechanosensory encoding: skin displacement at touch receptors, mechanotransduction and action-potential initiation.
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