Sentence examples for by sequential passage of from inspiring English sources

The hADSCs were purified by sequential passage of SVF cells in a culture medium specific for the mesenchymal cell lineage.

Cell cycle analysis of MEFs during serum starvation and release showed the expected outcome, namely efficient G0/G1 arrest (∼93%), followed by sequential passage of cells through early/mid/late S phase into G2/M phase.

The strategy presented here exploits a comprehensive lineage of genetically related parasites, comprising drug-resistant mutants previously generated [ 13- 16] by the sequential passage of parasites in mice treated with individual drugs (including artemisinin and artesunate) and the intermittent fixation of mutations by cloning.

High-titre adenoviral stocks were generated by sequential passage in HEK-293A cells according to the manufacturer's instructions (Stratagene).

Guinea pig has been infected with ZEBOV by sequential passage and presented EVD-like symptoms such as fever, anorexia, dehydration [ 43], and demonstrated progressive prolongation of the PT and aPTT [ 44] but rash does not develop in these animals [ 42].

It appears growth cones can detect the sequential passage of a glial Ca2+ wave from its activity-proximal side to its distal side, however the physical mechanism by which these small temporal and spatial gradients are detected by the growth cone is unknown.

Independent replicates of A549 (n = 8), UKY-29 (n = 3), H3255 (n = 3), PC-9 (n = 5), and H1650 (n = 6) were generated by using sequential passages of the cell populations.

S180 cells were maintained in the ascitic form by sequential passages in ICR mice.

Immortalized MEFs (MEFi) were prepared from primary MEFs by sequential passages as for 3T3 cell line establishment.

A bone-seeking clone (MDA-231BO) of the human breast cancer cell line MDA-231 was constructed by sequential passages in mice and the metastatic cells collected from bone [ 17].

Cells were lysed by sonication in lysis buffer [50 mM HEPES (pH 7.5), 200 mM sodium chloride, 5 mM β-mercaptoethanol, 0.1 mM PMSF, and 0.25% DDM], and GST PIR1 fusion proteins were purified by sequential passages over GST beads (GenScript).