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BAL was performed by sequential instillation of five aliquots of 30 mL non-bacteriostatic saline solution at room temperature.
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BAL was performed by sequential instillation and aspirations of three aliquots of 60 mL of warmed sterile 0.9% saline.
Bronchoalveolar lavage (BAL) was performed by sequential instillation and aspirations of three aliquots of 60 ml of warmed sterile 0·9% saline.
Bilateral bronchoalveolar lavage (BAL) was performed by two consecutive instillations of 1.0 ml of PBS.
In detail, broncho-alveolar lavage was performed by instillation of two sequential aliquots of 1 mL/kg (up to a maximum of 5 mL) 0.9% NaCl warmed at 37°C, into the endotracheal tube, followed by three respiratory cycles.
Titrations were performed by sequential addition of ligand to enzyme.
ALI was induced by intratracheal instillation of LPS.
ALI was induced by intratracheal instillation of HCl (0.1mol/L) followed by instillation of LPS from Escherichia coli O55 B5 (30µg/g body weight) 2h later.
IAH (20 mm Hg) was generated by intraperitoneal instillation of warmed saline.
Colitis was induced in rats by intracolonic instillation of trinitrobenzene sulfonic acid (TNBS).
Colitis was induced in rats by intracolonic instillation of dinitrobenzene sulfonic acid.
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