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Subsequently, multipoint linkage analysis was performed to determine the most significant position of the SNPs in each linkage group by sequential insertion of SNPs using the ALL option.
ss-GFP-FM4-CD8 (CD8lumenal)was generated by sequential insertion of GFP and CD8 encoding sequences into a pC4-FM4 backbone vector (ARIAD), using XbaI/SpeI compatibility, and BamH1 restriction site.
CD8lumenal (ss-GFP-FM4-CD8 and ss-DsRed-FM4-CD8) were generated by sequential insertion of GFP (or DsRed), and CD8 encoding sequences into a pC4 ss-FM4 backbone vector (ARIAD), using XbaI/SpeI compatibility and BamH1 restriction site.
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Although these tandem SINEU-3 copies were reported as SINEU-3B in our previous analysis (Green et al. 2014), they are more likely generated by a sequential insertion of two SINEU-3 copies and should not be considered as a subfamily of SINEU-3.
We propose that the long strings of rhs genes and gene fragments that have been observed are created by sequential insertion events, whereby existing C-terminal tips are displaced with non-homologous alternatives.
Persistent strictures were treated by repeated dilation and sequential insertion of multiple stents.
Persistent strictures will be treated by repeated endoscopic dilations and sequential insertion of new stents for a maximal period of 1 year, after which stenting must be stopped.
By this means, the Rhs protein can drive the sequential insertion of heterogeneous C-terminal sequences into the hot spot [ 36].
We demonstrate, through a series of examples, optimal topology achieved through sequential insertion of a heterogeneity of fixed shape and optimization of its configuration (location, orientation and scale).
One repeat was amplified from mouse genomic DNA and was TOPO-cloned and PCR-amplified to yield IBRE fragments flanked by SacI- MluI, MluI- XmaI, XmaI- XhoI and XhoI- BglII, respectively, to allow for sequential insertion of the fragments into the pTA-based vectors to generate the pIBRE4-TA-Cerulean.
This has led to the suggestion 38) that simultaneous incorporation of both atoms of O2, as dictated by Scheme 2, does not occur but that instead sequential (stepwise) insertion of oxygen into the substrate and accompanying oxidation to ferryl heme is the preferred route, as depicted in Scheme 5A.
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