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The resulting gels were purified by sequential extractions with THF, THF/water mixture, water, water/acetone mixture, and finally acetone for 1 day.

Serum samples were prepared for FI-FTICR-MS by sequential extractions with 1 1 5 volumes of 1% ammonium hydroxide and ethyl acetate (EtOAc) three times.

The M. alba crude extract was re-suspended with water (150 mL), followed by sequential extraction with ethyl acetate (9 × 150 mL) and butanol (6 ×150 mL).

Prior to analyses, the sediments were fractionated by sequential extraction with heptane, toluene, and tetrahydrofuran.

Crude fiber was measured by sequential extraction with diluted acid and alkali (method 978.10) and Ca and P by spectrophotometry (methods 968.08 and 965.17) as indicated by AOAC (2000).

Fig. 1 Mass fractions (mg/g sediment) and phase associations of mobile elements in the sampled rocks, as determined by sequential extraction with 1 g of rock and 30 mL of solvent.

Composted PL samples were fractionated into readily plant-available P, labile inorganic P (another plant-available fraction), sesquioxide-associated (Fe oxide and Al oxide) inorganic P- and Ca-associated P by sequential extraction with de-ionized water, 0.5 M NaHCO3 (pH 8.5), 0.1 M NaOH, and 1 M HCl.

The soluble content was determined by sequential extraction with different organic solvents.

Propolis (90 g) was initially extracted by 95% (v/v) ethanol and then solvent partitioned by sequential extractions of the crude ethanolic extract with 40% (v/v) MeOH, CH2Cl2 and hexane.

Therefore, no potential contaminants to local groundwater are expected as revealed by sequential extractions.

Figure 3a shows the strong enrichment of the Zera-DsRed protein in the PB extract before the sequential extractions with a pink color given by the DsRed.