Sentence examples for by sequential elution with from inspiring English sources
The lipid was eluted from the column by sequential elution with 100 ml each of 1, 1, 1-trichloroethane, acetone, and methanol and the fractions collected.
Proteins were eluted from the matrix by sequential elution with the peptides GKKRARAADLE ('RARA'), GKKRVRWADLE ('RVRW'), and finally, 3M sodium thiocyanate.
The extract was then adsorbed onto a 10-ml Bond Elut SI solid-phase (SP) fractionation cartridge (Agilent Technologies, Sweden), followed by sequential elution with cyclohexane, EtOAc, and methanol (MeOH).
The column was preconditioned by sequential elution with 200mL of methanol and 200mL of buffered methanol.
Phosphopeptides can be separated according to their phosphorylation level by sequential elution with an increasing concentration of potassium hydrogen phosphate in the elution buffer (Mamone et al. 2010).
Different eluents gave different phosphopeptide spectra, showing the usefulness of carrying out sequential elution with various elution buffers rather than relying on just one (Kyono et al. 2008).
Soluble extracts were prepared from whole wild type C57Bl/6 mouse brain and purified on a diethylaminoethyl (DEAE Fast Floww column (GE Healthcare Life Sciences, Pittsburgh, PA) with sequential elution with increasing concentrations of NaCl as described [ 24, 25].
The recombinant polymer was purified from the clear supernatant with the HisTrap HP nickel column using sequential elutions with buffers containing imidazole.
[65 69] In all these prior studies, the resin was used to sequester the dissolved species, followed by a sequential elution to allow for the analyses of the different types of sulfur species.
Many proteins were found both in ATP- and NaCl-eluted fractions, some of them possibly due to the sequential elution first by ATP and second by NaCl.
